Vilon (20 mg Vial) Protocol

Vilon Chart, Vitality Labs

Vilon is dosed at 67 mcg–667 mcg daily via subcutaneous Procedure in educational protocols. A 20 mg vial reconstituted with bacteriostatic water yields about 6.67 mg/mL. This information is for research and educational use only.

Vilon (Lys‑Glu) is a synthetic immunoregulatory dipeptide consisting of lysine and glutamic acid residues. Preclinical research indicates it may enhance immune markers including CD5+ lymphocytes and interleukin‑2 expression in thymic and splenic cell cultures. This educational protocol presents a pulsed subcutaneous approach (5 consecutive days per 4‑week cycle) using a practical dilution for clear insulin‑syringe measurements.

Educational guide for Solution and pulsed dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Schedule: Procedureonce daily subcutaneously for 5 consecutive days, then rest for the remainder of a 4‑week cycle. Repeat monthly.

Phase / Cycle	Daily Dose (mcg)	Units (per Procedure ) (mL)
Cycle 1, Day 1	67 mcg (0.067 mg)	1 unit (0.01 mL)
Cycle 1, Day 2	133 mcg (0.133 mg)	2 units (0.02 mL)
Cycle 1, Day 3	200 mcg (0.20 mg)	3 units (0.03 mL)
Cycle 1, Day 4	267 mcg (0.267 mg)	4 units (0.04 mL)
Cycle 1, Day 5	333 mcg (0.33 mg)	5 units (0.05 mL)
Cycle 2+ (Days 1–5)	333–667 mcg (0.33–0.67 mg)	5–10 units (0.05–0.10 mL)

For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.

Solution Preparation Steps

Plan based on an 8–16 week pulsed protocol (5 Procedure days per 4‑week cycle).

Concise summary of the pulsed regimen.

Goal: Support immune modulation and thymic function markers based on preclinical observations.
Schedule: 5 consecutive days of subcutaneous Procedure per 4-week cycle; repeat for 2–4 cycles.
Dose Range: 67–667 mcg daily with gradual titration during the first cycle.
Solution Preparation 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate low-volume measurements.
Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

Suggested pulsed titration approach.

Start: 67 mcg (1 unit) on Day 1; increase by ~67 mcg (1 unit) each day during first cycle.
Target: 333–667 mcg (5–10 units) daily by Cycle 2 onward.
Frequency: Once per day (subcutaneous) for 5 consecutive days per cycle.
Cycle Length: 5 days on, ~23 days off (4-week cycle); run 2–4 cycles.
Timing: Any consistent time; rotate Procedure sites.

Proper storage preserves peptide quality.

Lyophilized: Store at −20 °C (−4 °F) short-term or −80 °C (−112 °F) long-term in dry, dark conditions; minimize moisture exposure.
Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within ~1 week or prepare aliquots and freeze to extend stability.
Allow vials to reach room temperature before opening to reduce condensation uptake.

Practical considerations for consistency and safety.

Use new sterile insulin syringes (30‑ or 50‑unit recommended for low‑volume accuracy); dispose in a sharps container.
Rotate Procedure sites (abdomen, thighs, upper arms) to reduce local irritation.
Procedure slowly; wait a few seconds before withdrawing the needle.
Document daily dose and site rotation to maintain consistency across cycles.

Vilon belongs to a class of short bioregulatory peptides developed through Russian gerontology research. In vitro studies demonstrate that the Lys-Glu dipeptide can modulate lymphocyte populations and cytokine gene expression. Specifically, Vilon has been observed to increase CD5+ cell counts in thymus cell cultures and upregulate interleukin-2 (IL-2) mRNA expression in splenocyte preparations. Additional preclinical work suggests effects on TGF-β1 and vascular permeability in renal models. The pulsed 5-day monthly regimen is analogized from related short peptide protocols such as Epitalon.

Observations from preclinical literature.

Supports immune cell marker expression (CD5+ lymphocytes) and IL‑2 gene activity in cell culture models.
May modulate inflammatory cytokines such as TGF‑β1 under experimental renal stress conditions.
Early mouse studies suggest potential effects on tumor growth inhibition and lifespan extension, though human data remain limited.
Generally well tolerated in preclinical settings; occasional mild Procedure‑site reactions (redness/itch) may occur with subcutaneous administration.

Complementary strategies for best outcomes.

General subcutaneous guidance from clinical best‑practice resources.

Clean the vial stopper and skin with alcohol; allow to dry.
Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
Do not aspirate for subcutaneous Procedure; Procedure slowly and steadily.
Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy

We recommend Vitality Labs Peptides for high‑purity Vilon (20 mg).

Why Vitality Labs Peptides?

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

Sevostianova NN et al. (2013)

— Immunomodulating effects of Vilon and its analogue in the culture of human and animal thymus cells

Kazakova TB et al. (2002)

— In vitro effect of short peptides on expression of interleukin‑2 gene in splenocytes

Gavrisheva NA et al. (2005)

— Effect of Vilon peptide on TGF‑β1 and vascular permeability in chronic renal failure

Kniaz’kin IA et al. (2002)

— Effect of a peptide Vilon on immune aging (Adv Gerontol)

Araj SK et al. (2025)

— Overview of Epitalon—highly bioactive pineal tetrapeptide with promising properties (Int J Mol Sci)

Chernyak EY et al. (2000)

— Synthetic dipeptide Vilon inhibits tumor growth and prolongs life in mice (Dokl Biol Sci)

Avolio F et al. (2022)

— Peptides regulating proliferative activity and inflammatory pathways in THP‑1 macrophages (Int J Mol Sci)

Wong AKC et al. (2024)

— Procedure techniques to reduce subcutaneous heparin complications (J Adv Nurs)

MedlinePlus (NIH)

— Subcutaneous (SQ) Procedure: patient information

Merck KGaA (Millipore Sigma)

— Handling and storage guidelines for peptides and proteins

CDC

— Vaccine administration: subcutaneous route (angle/site; no aspiration)

NCBI Bookshelf

— Best practices for Procedure (asepsis, preparation, and administration)

Vitality Labs Peptides

— Vilon (20 mg) product page (quality and batch documentation)